 หัวเรื่อง:การคัดเลือกแคลลัสของข้าวพันธุ์ขาวดอกมะลิ 105 ให้ทนทานต่อสารกำจัดวัชพืชไกลฟอเสท ผู้เขียน: Suriyan Cha-um, Srisom Surawattananon, Kramolpun Namwongprom,  นายเฉลิมชัย วงศ์วัฒนะ, อาจารย์ สื่อสิ่งพิมพ์:pdf AbstractCalli of KDML105 were selected for glyphosate-tolerance on solidified 0.25% Phytagel?-solidified MS (Murashige and Skoog, 1962) selection medium (MS salts and vitamins, 1 mgl-1 kinetin, and 10-4 M glyphosate) at 25?2 ?C, 70?5 ?mol m-2s-1 photosynthetic photon flux density (PPFD), and 16 hd-1 photoperiod. Calli fresh weight were decreased in selection medium more than in glyphosate-free medium. The selected calli at 4 and 8 weeks (S4 and S8) could be regenerated on the glyphosate-free medium. Plantlets were propagated by tissue culture technique and treated with 0, 10-5, 5?10-5 and 10-4 M glyphosate in vitro. The height of S4 and S8 plantlets was decreased with increasing glyphosate concentrations in culture media. The number of tillers and number of roots were increased in culture media supplemented with 10-5 M glyphosate. The S8 plantlets could survived on the culture supplemented with 5?10-5 M glyphosate. Increasing the height of plantlets indicated high survival percentage under glyphosate-stressed condition. Tolerant plants were transferred to pots and treated with 0, 250, 500, 1000 and 2000 mgl-1 glyphosate. The growth of plants was inhibited and phytotoxicity was observed when treated with high glyphosate concentrations. Increasing glyphosate concentrations resulted in lesser toxicity in S8 plantlets than in S4 plantlets. |
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 Researcherดร. จรีรัตน์ มงคลศิริวัฒนา, ผู้ช่วยศาสตราจารย์ที่ทำงาน:ภาควิชาวิทยาศาสตร์และนวัตกรรมชีวภาพ คณะศิลปศาสตร์และวิทยาศาสตร์ สาขาที่สนใจ:พันธุวิศวกรรม epigenetics and small RNA , การโคลนยีนและการถ่ายยีนในพืช , การควบคุมและการแสดงออกของยีน , ชีวสารสนเทศ Resume |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Accumulation of Glycinebetaine and Betaine Aldehyde Dehydrogenase Activity in Eucalyptus camaldulensis Clone T5 Under in vitro Salt Stress) ผู้เขียน:ศยามล สิทธิสาร, ดร.ปุณฑริกา หะริณสุต, รองศาสตราจารย์, Somchai Pornbunlualap, Suriyan Cha-um สื่อสิ่งพิมพ์:pdf AbstractA widely distributed adaptation to counteract abiotic stress in plant is an accumulation of compatible organic solutes. There are many different types of salts and almost an equally diverse set of salt-tolerant defense mechanisms. Glycinebetaine (GB) is a major organic osmolyte that accumulates in a variety of plant species in response to salt stress. This research focused on the elucidation of the GB accumulation in the E. camaldulensis clone T5, under NaCl salinity. The results showed that there was a high correlation between GB accumulation and NaCl concentration, for a range of 0 to 500 mM NaCl. The biosynthesis of GB in the plant involved betaine aldehyde dehydrogenase (BADH) as a key enzyme, which also increased as the concentration of NaCl increased. The BADH protein contents in the extract were analyzed by SDS-PAGE gel. The result showed BADH protein expression with molecular mass 60 kDa. BADH activity was correlated with an accumulation of GB in response to salt stress. Total chlorophyll content in multiple shoots was unchanged in the first 13 days with 200 mM NaCl. |
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ผลงานตีพิมพ์ในวารสารวิชาการCombining high-throughput phenotyping with overall growth measurements of indica rice (Oryza sativa L spp. indica) cultivars over the whole life cycleผู้แต่ง:Cattarin Theerawitaya, Ms.Cattleya Chutteang, Assistant Professor , Dr.Anuruck Arunyanark, Assistant Professor , Dr.Chanate Malumpong, Associate Professor , Narubodin Kwangern, Nattapol Rachsapa, Piyanan Pipatsitee, Patchara Prasertkul, Suriyan Cha-um, Theerayut Toojinda, วารสาร: 
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